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KMID : 1059520190630050335
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Journal of the Korean Chemical Society
2019 Volume.63 No. 5 p.335 ~ p.341
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Analysis of Low Molecular Weight of Seleno compounds in Selenium-Fortified Spirulina
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Ji Young
Lee Jung-Suk
Han Young-Seok
Pak Yong-Nam
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Abstract
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Spirulina was cultured in Selenium solution and the total concentration was determined with isotope dilution technique. Low-molecular-weight-Selenium species for the water extract of Spirulina were separated and quantified with HPLC ICP/ MS. Water extraction was used first and then protein enzyme (protease XIV) was used to digest and extract for the Se species in both water extract and residue. The total Se was 414.9 ± 4.0 μg g?1 and 77% existed in water extract while 22% remained in residue. Se species in supernatant was mostly inorganic selenate (222.7 μg g?1). After hydrolysis of protein, SeCys (15.20 μg g?1) and SeMet (12.13 μg g?1) were found. In residue, SeCys and SeMet were found with little inorganic Se. After protein hydrolysis of residue, more of Selenoamino acids SeCys (9.35 μg g?1) and SeMet (18.23 μg g?1) in addition to MeSeCys (1.5 μg g?1) were found. It is thought that inorganic selenium is mostly adsorbed on the surface of spirulina and can be easily removed by a simple distilled water extraction while most of organo-seleniums are remained in residue.
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KEYWORD
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Selenium speciation, Selenium-fortified Spirulina, Isotope dilution, HPLC-ICP/MS
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